( We provide with TEK qPCR primers for gene expression analysis, HP100881 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10700-ACG|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10700-ACR|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10700-CF|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10700-CH|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10700-CM|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10700-CY|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA(克隆载体)||HG10700-M|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10700-NF|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10700-NH|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10700-NM|
|人 TEK / Tie2 ORF mammalian expression plasmid, N-HA 标签||HG10700-NY|
|人 Tie2/CD202b/TEK 基因ORF全长cDNA克隆(表达载体)||HG10700-UT|
TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.