|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10569-ACG|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10569-ACR|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10569-CF|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10569-CH|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10569-CM|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10569-CY|
|人 TGFBI 基因ORF全长cDNA(克隆载体)||HG10569-M|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10569-M-F|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10569-NF|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10569-NH|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10569-NM|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10569-NY|
|人 TGFBI 基因ORF全长cDNA克隆(表达载体)||HG10569-UT|
TGFBI is an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. TGFBI plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. TGFBI is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in TGFBI are associated with multiple types of corneal dystrophy. TGFBI can bind to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, TGFBI may be involved in endochondral bone formation. Loss of the TGFBI is sufficient to induce specific resistance.