|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13750-ACG|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13750-ACR|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13750-CF|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13750-CH|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13750-CM|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13750-CY|
|人 TMEM25 基因ORF全长cDNA(克隆载体)||HG13750-G|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13750-NF|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13750-NH|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13750-NM|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13750-NY|
|人 TMEM25 基因ORF全长cDNA克隆(表达载体)||HG13750-UT|
TMEM25 is a novel member of the immunoglobulin superfamily. Immunoglobulin superfamily members are implicated in immune responses, growth factor signaling, and cell adhesion. TMEM25 contains 1 Ig-like (immunoglobulin-like) domain and is a target of pharmacogenomics in the field of oncology and regenerative medicine. TMEM25 isoform 1, consisting of exons 1-9, encoded a 366-aa transmembrane protein. TMEM25 isoform 2, consisting of exons 1-4 and 6-9, encoded a 322-aa secreted protein. TMEM25 mRNA was expressed in brain, including cerebellar cortex and hippocampus, as well as in neuroblastoma, brain tumors, and gastric cancer. Human TMEM25 gene was located at the 11q23.3 oncogenomic recombination hotspot around the MLL amplicon and the neuroblastoma deleted region.