|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG16078-ACG|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG16078-ACR|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG16078-CF|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG16078-CH|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG16078-CM|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG16078-CY|
|人 TNFRSF11A 基因ORF全长cDNA(克隆载体)||HG16078-G|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG16078-NF|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG16078-NH|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG16078-NM|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG16078-NY|
|人 TNFRSF11A 基因ORF全长cDNA克隆(表达载体)||HG16078-UT|
TNFRSF11A is a member of the TNF-receptor superfamily. In mouse, it is also known as CD265. TNFRSF11A contains 4 TNFR-Cys repeats and is widely expressed with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. It is an essential mediator for osteoclast and lymph node development. TNFRSF11A and its ligand are important regulators of the interaction between T cells and dendritic cells. It can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. Defects in TNFRSF11A can cause familial expansile osteolysis (FEO). FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. Defects in TNFRSF11A also can cause Paget disease of bone type 2 (PDB2). PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 which characterized by abnormally dense bone, due to defective resorption of immature bone.