Osteoprotegerin cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG10271-NF
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Osteoprotegerin cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
1206 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 912T/C not causing the amino acid variation.
描述
Full length Clone DNA of Human tumor necrosis factor receptor superfamily, member 11b with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-SP-N-FLAG
限制性酶切位点
KpnI (two restriction sites) + XbaI (6kb+1.07kb+0.17kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
Osteoprotegerin cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Osteoprotegerin cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images
Human Osteoprotegerin/TNFRSF11B Gene Plasmid Map 5676
Human Osteoprotegerin/TNFRSF11B Gene Expression validated Image 16359
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
Osteoprotegerin cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
MGC29565 cDNA ORF Clone, Human;OCIF cDNA ORF Clone, Human;OPG cDNA ORF Clone, Human;PDB5 cDNA ORF Clone, Human;TR1 cDNA ORF Clone, Human
Osteoprotegerin Background Information

Osteoprotegerin or TNFRSF11B is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. Osteoprotegerin/TNFRSF11B acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. This protein may inhibit the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. Osteoprotegerin/TNFRSF11B also play a role in preventing arterial calcification, act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.

Full Name
tumor necrosis factor receptor superfamily member 11b
References
  • Collin-Osdoby P. (2005) Regulation of vascular calcification by osteoclast regulatory factors RANKL and osteoprotegerin. Circ Res. 95 (11): 1046-57.
  • Boyce BF, et al. (2007) Biology of RANK, RANKL, and osteoprotegerin. Arthritis Res. Ther. 9 Suppl 1: S1.
  • Blázquez-Medela AM, et al. ( 2011) Osteoprotegerin and diabetes-associated pathologies. Curr Mol Med. 11 (5): 401-16.
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