|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10505-ACG|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10505-ACR|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10505-CF|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10505-CH|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10505-CM|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10505-CY|
|人 TPSB2 基因ORF全长cDNA(克隆载体)||HG10505-M|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10505-NF|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10505-NH|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10505-NM|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10505-NY|
|人 TPSB2 基因ORF全长cDNA克隆(表达载体)||HG10505-UT|
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1, and fall into two groups, α and β. β-tryptases appear to be the main isoenzymes expressed in mast cells, whereas α-tryptases predominate in basophils. Tryptase is unique in two respects: it is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known endogenous proteinase inhibitors because of the unique arrangement of the active sites. Additionally, tryptase family genes have an intron immediately upstream of the initiator codon which separates the transcription initiation site from protein coding sequence, and this feature is characteristic of tryptases. β-tryptases existing in three isoforms (β1,β2,β3) are released in secretory granules, and have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. It has been reported that β-tryptase selectively cleaves ASM-derived eotaxin and RANTES and abrogates their chemotactic activities.