TPST1 qPCR Primer Pairs, Human

Cat: HP101142
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TPST1 qPCR Primer Pairs, Human 基本信息
Target Details
种属:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
运输:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
TPST1 qPCR Primer Pairs, Human Alternative Names
TANGO13A qPCR Primer Pairs, Human
TPST1 Background Information

Protein-tyrosine sulfotransferase 1, also known as Tyrosylprotein sulfotransferase 1 and TPST1, is a single-pass type I I membrane protein which belongs to the protein sulfotransferase family. Tyrosine O-sulfation is a common posttranslational modification of proteins in all multicellular organisms. This reaction is mediated by a Golgi enzyme activity called tyrosylprotein sulfotransferase (TPST) that catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to tyrosine residues within acidic motifs of polypeptides. Tyrosine O-sulfation has been shown to be important in protein-protein interactions in several systems. Tyrosine sulfation is mediated by one of two Golgi isoenzymes, called tyrosylprotein sulfotransferases (TPST-1 and TPST-2). A relatively small number of proteins are known to undergo tyrosine sulfation, including certain adhesion molecules, G-protein-coupled receptors, coagulation factors, serpins, extracellular matrix proteins, and hormones. TPST1 is a human tyrosylprotein sulfotransferase that uses 3'phosphoadenosine-5'phosphosulfate (PAPS) to transfer the sulfate moiety to proteins predominantly designated for secretion. TPST1 bears N-linked glycosyl residues exclusively at position Asn60 and Asn262. TPST1 and TPST2 have distinct biological roles that may reflect differences in their macromolecular substrate specificity.

Full Name
tyrosylprotein sulfotransferase 1
References
  • Ouyang Y.-B.et al., 1998, Proc. Natl. Acad. Sci. USA. 95: 2896-901.
  • Ouyang,Y.B. et al., 2002,J Biol Chem. 277 (26):23781-7.
  • Hoffhines, A.J. et al., 2006, J Biol Chem. 281 (49):37877-87.
  • Goettsch,S. et al., 2006, J Mol Biol. 361 (3):436-49.
  • Westmuckett, A.D. et al., 2008, Gen Comp Endocrinol. 156 (1):145-53.
  • TPST1 qPCR Primer Pairs, Rhesus, Related Products

    Product Name Catalog#(PDF)
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    TPST1 cDNA ORF Clone, Rhesus, C-His tag CG90690-CH
    TPST1 cDNA ORF Clone, Rhesus, N-His tag CG90690-NH
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    TPST1 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    TPST1 cDNA ORF Clone in Cloning Vector, Rat RG80470-U
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    TPST1 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80470-ACG
    TPST1 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80470-ACR
    TPST1 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80470-CF
    TPST1 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80470-NF
    TPST1 cDNA ORF Clone, Rat, C-His tag RG80470-CH
    TPST1 cDNA ORF Clone, Rat, N-His tag RG80470-NH
    TPST1 cDNA ORF Clone, Rat, C-HA tag RG80470-CY
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    TPST1 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    TPST1 cDNA ORF Clone in Cloning Vector, Mouse MG50617-M
    TPST1 cDNA ORF Clone, Mouse, untagged MG50617-UT
    TPST1 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50617-ACGLN
    TPST1 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50617-ACG
    TPST1 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50617-ACR
    TPST1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50617-CF
    TPST1 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50617-NF
    TPST1 cDNA ORF Clone, Mouse, C-His tag MG50617-CH
    TPST1 cDNA ORF Clone, Mouse, N-His tag MG50617-NH
    TPST1 cDNA ORF Clone, Mouse, C-HA tag MG50617-CY
    TPST1 cDNA ORF Clone, Mouse, N-HA tag MG50617-NY
    TPST1 cDNA ORF Clone, Mouse, C-Myc tag MG50617-CM
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    TPST1 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    TPST1 cDNA ORF Clone in Cloning Vector, Human HG11258-M
    TPST1 cDNA ORF Clone, Human, untagged HG11258-UT
    TPST1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11258-ACGLN
    TPST1 cDNA ORF Clone, Human, C-GFPSpark® tag HG11258-ACG
    TPST1 cDNA ORF Clone, Human, C-OFPSpark® tag HG11258-ACR
    TPST1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11258-CF
    TPST1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11258-NF
    TPST1 cDNA ORF Clone, Human, C-His tag HG11258-CH
    TPST1 cDNA ORF Clone, Human, N-His tag HG11258-NH
    TPST1 cDNA ORF Clone, Human, C-HA tag HG11258-CY
    TPST1 cDNA ORF Clone, Human, N-HA tag HG11258-NY
    TPST1 cDNA ORF Clone, Human, C-Myc tag HG11258-CM
    TPST1 cDNA ORF Clone, Human, N-Myc tag HG11258-NM
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