TRAIL Matched ELISA Antibody Pair Set,Human

1/1
Price:
Size:
Number:

TRAIL Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
TRAIL Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human TRAIL
线性范围
31.25-2000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 0.4 mg/mL of mouse anti-Human TRAIL/TNFSF10 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10409-MM03)
2. Detection Antibody 0.1 mg/mL of rabbit anti-Human TRAIL/TNFSF10 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 25(KW10AP902-1) ng of recombinant Human TRAIL/TNFSF10. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
产品概述
This TRAIL Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human TRAIL . It contains Human TRAIL capture antibody, Human TRAIL detector antibody (HRP) and a highly purified E. coli-expressed recombinant Human TRAIL protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

TRAIL Matched ELISA Antibody Pair Set,Human 图片

TRAIL Matched ELISA Antibody Pair Set,Human: 别称

Apo-2L Matched ELISA Antibody Pair Set, Human; APO2L Matched ELISA Antibody Pair Set, Human; CD253 Matched ELISA Antibody Pair Set, Human; TL2 Matched ELISA Antibody Pair Set, Human; TRAIL Matched ELISA Antibody Pair Set, Human

TRAIL 背景信息

Tumor necrosis factor ligand superfamily member 1 (TNFSF1), also known as TNF-related apoptosis-inducing ligand (TRAIL), Apo-2 ligand, and CD253, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TNFSF1 / Apo-2L / CD253 functions as a ligand that induces the process of cell death called apoptosis. TNFSF1 / TRAIL shows homology to other members of the tumor necrosis factor superfamily. As one member of the cluster of differentiation system, TNFSF1 / CD253 is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion TNFSF1 / Apo-2L / CD253 / TRAIL binds to several members of TNF receptor superfamily including TNFRSF1A / TRAILR1, TNFRSF1B / TRAILR2, TNFRSF1C / TRAILR3, TNFRSF1D / TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TNFSF1 / TRAIL may be modulated by binding to the decoy receptors TNFRSF1C / TRAILR3, TNFRSF1D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8 / JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
全称
tumor necrosis factor (ligand) superfamily, member 10
参考文献
  • Song C, et al. (2005) TRAIL (CD253), a new member of the TNF superfamily. J Biol Regul Homeost Agents. 19(1-2): 73-7.
  • Kuribayashi K, et al. (2008) TNFSF10 (TRAIL), a p53 target gene that mediates p53-dependent cell death. Cancer Biol Ther. 7(12): 2034-8.
  • Wiley SR, et al. (1995) Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity. 3(6): 673-82.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后