( We provide with Tie1 qPCR primers for gene expression analysis, HP100530 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 Tie1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10509-ACG|
|人 Tie1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10509-ACR|
|人 Tie1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10509-CF|
|人 Tie1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10509-CH|
|人 Tie1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10509-CM|
|人 Tie1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10509-CY|
|人 Tie1 基因ORF全长cDNA(克隆载体)||HG10509-M|
|人 Tie1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10509-NF|
|人 Tie1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10509-NH|
|人 Tie1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10509-NM|
|人 Tie1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10509-NY|
|人 Tie1 基因ORF全长cDNA克隆(表达载体)||HG10509-UT|
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 also known as Tie1 is an angiopoietin receptor and is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. The receptor tyrosine kinase Tie1 is expressed primarily in vascular endothelial cells. The receptor has also been detected in epithelial tumours in breast, thyroid and gastric cancers and in tumour cell lines where it appears as a 45 kDa truncated receptor fragment. Tie1 promotes endothelial cell survival, but other studies have suggested that the Tie1 kinase has little to no activity. Embryos deficient in Tie1 failed to establish structural integrity of vascular endothelial cells, resulting in oedema and subsequently localized haemorrhage. Tie1 is significantly higher in human aortic endothelial cells than in human umbilical vein endothelial cells. Additionally, attachment of cells of monocytic lineage to endothelial cells is also enhanced by Tie1 expression. Collectively Tie1 has a proinflammatory property and may play a role in the endothelial inflammatory diseases such as atherosclerosis.