快速订购

人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签

  • Rat TFRC ORF mammalian expression plasmid, C-Flag tag
  • Human Tyrosine Hydroxylase transcript variant 2 ORF mammalian expression plasmid, C-Flag tag
产品数据评论相关产品实验方法
 TH cDNA Clone产品信息
NCBI注册码:NM_000360.3
参考序列ORF长度:1494bp
cDNA基因描述:Full length Clone DNA of Homo sapiens tyrosine hydroxylase, transcript variant 2 with C terminal Flag tag.
分子别称:TYH, TH
分子种属:Human
载体:pCMV3-C-FLAG
质粒:pCMV3-TH-Flag
限制性酶切位点:KpnI + XbaI (two restriction sites) (6kb + 0.34kb + 1.21kb)
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with TH qPCR primers for gene expression analysis, HP100878 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 TH 基因质粒图
Human Tyrosine Hydroxylase transcript variant 2 ORF mammalian expression plasmid, C-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签 on other vectors
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10684-ACG1950
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10684-ACR1950
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10684-ANG1950
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10684-ANR1950
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10684-CF1770
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10684-CH1770
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10684-CM1770
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10684-CY1770
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA(克隆载体)HG10684-M760
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10684-NF1770
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10684-NH1770
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10684-NM1770
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10684-NY1770
人 TH / Tyrosine Hydroxylase transcript variant 2 基因ORF全长cDNA克隆(表达载体)HG10684-UT1770
 点击了解更多载体信息
Product nameProduct name
研究背景

Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

参考资料
  • Daubner SC, et al. (2011) Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys. 508(1): 1-12.
  • Dunkley PR, et al. (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem. 91(5): 1025-43.
  • Haavik J, et al. (1998) Tyrosine hydroxylase and Parkinson's disease. Mol Neurobiol. 16(3): 285-309.
  • Size / Price
    货号: HG10684-CF
    目录价: 
    单价:      (You Save: )

    Datasheet & Documentation

    Contact Us
      您曾浏览过的产品
        产品数据可能随批次变化,最新数据请详见随货说明书。
        请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”