Tyrosine Hydroxylase cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10684-CF

Contact us:

215-583-7898
order@sinobiological.com
Distributors

All Tyrosine Hydroxylase Reagents

CRO Services

Product recommended


All Tyrosine Hydroxylase Reagents

Tyrosine Hydroxylase cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
1494 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human tyrosine hydroxylase, transcript variant 2 with C terminal Flag tag.

质粒

启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI (two restriction sites) (6kb + 0.34kb + 1.21kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

Tyrosine Hydroxylase cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Tyrosine Hydroxylase cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images

Human TH / Tyrosine Hydroxylase Gene Plasmid Map 5734
Human TH / Tyrosine Hydroxylase Gene Expression validated Image 14371
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

Tyrosine Hydroxylase cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names

DYT14 cDNA ORF Clone, Human;DYT5b cDNA ORF Clone, Human;TYH cDNA ORF Clone, Human

Tyrosine Hydroxylase Background Information

Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

Full Name
tyrosine hydroxylase
References
  • Daubner SC, et al. (2011) Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys. 508(1): 1-12.
  • Dunkley PR, et al. (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem. 91(5): 1025-43.
  • Haavik J, et al. (1998) Tyrosine hydroxylase and Parkinson's disease. Mol Neurobiol. 16(3): 285-309.
  • Tyrosine Hydroxylase cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    Tyrosine Hydroxylase cDNA ORF Clone in Cloning Vector, Mouse MG50997-G
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, untagged MG50997-UT
    Tyrosine Hydroxylase Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50997-ACGLN
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50997-ACG
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, N-GFPSpark® tag MG50997-ANG
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50997-ACR
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, N-OFPSpark® tag MG50997-ANR
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50997-CF
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50997-NF
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, C-His tag MG50997-CH
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, N-His tag MG50997-NH
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, C-HA tag MG50997-CY
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, N-HA tag MG50997-NY
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, C-Myc tag MG50997-CM
    Tyrosine Hydroxylase cDNA ORF Clone, Mouse, N-Myc tag MG50997-NM
    Tyrosine Hydroxylase qPCR Primer Pairs, Mouse MP200962

    Tyrosine Hydroxylase cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    Tyrosine Hydroxylase cDNA ORF Clone in Cloning Vector, Human HG10684-M
    Tyrosine Hydroxylase cDNA ORF Clone, Human, untagged HG10684-UT
    Tyrosine Hydroxylase cDNA ORF Clone, Human, C-GFPSpark® tag HG10684-ACG
    Tyrosine Hydroxylase Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10684-ACGLN
    Tyrosine Hydroxylase cDNA ORF Clone, Human, N-GFPSpark® tag HG10684-ANG
    Tyrosine Hydroxylase cDNA ORF Clone, Human, C-OFPSpark® tag HG10684-ACR
    Tyrosine Hydroxylase cDNA ORF Clone, Human, N-OFPSpark® tag HG10684-ANR
    Tyrosine Hydroxylase cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10684-NF
    Tyrosine Hydroxylase cDNA ORF Clone, Human, C-His tag HG10684-CH
    Tyrosine Hydroxylase cDNA ORF Clone, Human, N-His tag HG10684-NH
    Tyrosine Hydroxylase cDNA ORF Clone, Human, C-HA tag HG10684-CY
    Tyrosine Hydroxylase cDNA ORF Clone, Human, N-HA tag HG10684-NY
    Tyrosine Hydroxylase cDNA ORF Clone, Human, C-Myc tag HG10684-CM
    Tyrosine Hydroxylase cDNA ORF Clone, Human, N-Myc tag HG10684-NM
    Tyrosine Hydroxylase qPCR Primer Pairs, Human HP100878
    info info
    添加购物车成功! 添加购物车失败!