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人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签

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Human USH1C cDNA Clone产品信息
NCBI注册码:NM_005709.2
参考序列ORF长度:1659bp
cDNA基因描述:Full length Clone DNA of Homo sapiens Usher syndrome 1C (autosomal recessive, severe), transcript variant 1 with C terminal Myc tag.
分子别称:PDZ73, AIE-75, DFNB18, PDZ-45, PDZ-73, NY-CO-37, NY-CO-38, ush1cpst, PDZ-73/NY-CO-38
分子种属:Human
载体:pCMV3-C-Myc
质粒:
限制性酶切位点:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签 on other vectors
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10613-ACG2070
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10613-ACR2070
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10613-ANG2070
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10613-ANR2070
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10613-CF1890
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10613-CH1890
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10613-CM1890
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10613-CY1890
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA(克隆载体)HG10613-M920
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10613-M-F1890
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10613-NF1890
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10613-NH1890
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10613-NM1890
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10613-NY1890
人 USH1C/Harmonin transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG10613-UT1890
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Product nameProduct name
研究背景

Harmonin, also known as Antigen NY-CO-38 / NY-CO-37, Autoimmune enteropathy-related antigen AIE-75, Protein PDZ-73, Renal carcinoma antigen NY-REN-3, Usher syndrome type-1C protein and USH1C, is a protein which is expressed in small intestine, colon, kidney, eye and weakly in pancreas. USH1C is expressed also in vestibule of the inner ear. USH1C contains 3 PDZ (DHR) domains. USH1C may be involved in protein-protein interaction. Defects in USH1C are the cause of Usher syndrome type 1C (USH1C), also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). Defects in USH1C are also the cause of deafness autosomal recessive type 18 (DFNB18) which is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

参考资料
  • Verpy, E. et al., 2000, Nat Genet. 26 (1):51-5.
  • Weil D., et al., 2003, Hum. Mol. Genet. 12:463-471.
  • Reiners,J. et al., 2005, Hum Mol Genet. 14 (24):3933-43.
  • Yan,D. et al., 2006, Mol Biol. 357 (3):755-64.
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