|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10808-ACG|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10808-ACR|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10808-CF|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10808-CH|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10808-CM|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10808-CY|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA(克隆载体)||HG10808-M|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10808-M-F|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10808-NF|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10808-NH|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10808-NM|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10808-NY|
|人 Uteroglobin/SCGB1A1 基因ORF全长cDNA克隆(表达载体)||HG10808-UT|
Uteroglobin (UG), also known as Secretoglobin 1A member 1 (SCGB1A1), Blastokinin, Clara cell secretor protein (CCSP) or Clara cell-specific 10-kDa protein (CC10), is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is mainly expressed in lung, with anti-inflammatory/immunomodulatory properties. Previous in vitro studies demonstrated that CCAAT/enhancer-binding proteins (C/EBPs) are the major transcription factors for the regulation of SCGB1A1 gene expression, whereas FOXA1 had a minimum effect on the transcription. Uteroglobin is a multifunctional protein with antiinflammatory/immunomodulatory properties. Uteroglobin inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, Uteroglobin manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities. The tissue-specific expression of the Uteroglobin gene is regulated by several steroid hormones, although a nonsteroid hormone, prolactin, further augments its expression in the uterus. Based on its anti-inflammatory and antiallergic properties, Uteroglobin is a potential drug target. The mechanism of Uteroglobin action is likely to be even more complex as it also functions via a putative receptor-mediated pathway.