VEGF B cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG10544-NF
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VEGF B cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
624 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human vascular endothelial growth factor B with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-SP-N-FLAG
限制性酶切位点
HindIII + XbaI (6kb + 0.65kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
VEGF B cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

VEGF B cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images
Cynomolgus CD19/B4/CVID3 Gene Plasmid Map 5623
Human VEGF-B Gene Expression validated Image 14448
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
VEGF B cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
VEGFL cDNA ORF Clone, Human;VRF cDNA ORF Clone, Human
VEGF B Background Information
Full Name
vascular endothelial growth factor B

VEGF B cDNA ORF Clone, Rat, Related Products

Product Name Catalog#(PDF)
VEGF B cDNA ORF Clone in Cloning Vector, Rat RG80114-G
VEGF B cDNA ORF Clone, Rat, untagged RG80114-UT
VEGF B cDNA ORF Clone, Rat, C-GFPSpark® tag RG80114-ACG
VEGF B Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80114-ACGLN
VEGF B cDNA ORF Clone, Rat, C-OFPSpark® tag RG80114-ACR
VEGF B cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80114-CF
VEGF B cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80114-NF
VEGF B cDNA ORF Clone, Rat, C-His tag RG80114-CH
VEGF B cDNA ORF Clone, Rat, N-His tag RG80114-NH
VEGF B cDNA ORF Clone, Rat, C-HA tag RG80114-CY
VEGF B cDNA ORF Clone, Rat, N-HA tag RG80114-NY
VEGF B cDNA ORF Clone, Rat, C-Myc tag RG80114-CM
VEGF B cDNA ORF Clone, Rat, N-Myc tag RG80114-NM
VEGF B qPCR Primer Pairs, Rat RP300109

VEGF B cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
VEGF B cDNA ORF Clone in Cloning Vector, Mouse MG50158-M
VEGF B cDNA ORF Clone, Mouse, untagged MG50158-UT
VEGF B Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50158-ACGLN
VEGF B cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50158-ACG
VEGF B cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50158-ACR
VEGF B cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50158-CF
VEGF B cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50158-NF
VEGF B cDNA ORF Clone, Mouse, C-His tag MG50158-CH
VEGF B cDNA ORF Clone, Mouse, N-His tag MG50158-NH
VEGF B cDNA ORF Clone, Mouse, C-HA tag MG50158-CY
VEGF B cDNA ORF Clone, Mouse, N-HA tag MG50158-NY
VEGF B cDNA ORF Clone, Mouse, C-Myc tag MG50158-CM
VEGF B cDNA ORF Clone, Mouse, N-Myc tag MG50158-NM
VEGF B qPCR Primer Pairs, Mouse MP200188

VEGF B cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
VEGF B cDNA ORF Clone in Cloning Vector, Human HG10544-M
VEGF B cDNA ORF Clone, Human, untagged HG10544-UT
VEGF B Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10544-ACGLN
VEGF B cDNA ORF Clone, Human, C-GFPSpark® tag HG10544-ACG
VEGF B cDNA ORF Clone, Human, C-OFPSpark® tag HG10544-ACR
VEGF B cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10544-CF
VEGF B cDNA ORF Clone, Human, C-His tag HG10544-CH
VEGF B cDNA ORF Clone, Human, N-His tag HG10544-NH
VEGF B cDNA ORF Clone, Human, C-HA tag HG10544-CY
VEGF B cDNA ORF Clone, Human, N-HA tag HG10544-NY
VEGF B cDNA ORF Clone, Human, C-Myc tag HG10544-CM
VEGF B cDNA ORF Clone, Human, N-Myc tag HG10544-NM
VEGF B qPCR Primer Pairs, Human HP100560
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