WTAP cDNA ORF Clone, Human, N-His tag

Cat: HG12018-NH

Contact us:

215-583-7898
order@sinobiological.com
Distributors

All WTAP Reagents

CRO Services

Product recommended


All WTAP Reagents

WTAP cDNA ORF Clone, Human, N-His tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
1191 bp
描述
Full length Clone DNA of Human Wilms tumor 1 associated protein with N terminal His tag.

质粒

启动子
Enhanced CMV promoter
载体
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

WTAP cDNA ORF Clone, Human, N-His tag Alternative Names

Mum2 cDNA ORF Clone, Human

WTAP Background Information

Wilms' tumor 1-associating protein (WTAP) was previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. WT1 was originally identified as a tumor suppressor for Wilms' tumor, but it is also overexpressed in a variety of cancer cells. The WTAP-WT1 axis in vascular cells suggest that WTAP is a vital and multifaceted regulator of vascular remodeling. WTAP has been suggested to function in alternative splicing, stabilization of mRNA, and cell growth. Knocking down endogenous WTAP increased Smooth muscle cells (SMCs) proliferation, because of increased DNA synthesis and G(1)/S phase transition, together with reduced apoptosis. These effects could be the result of WTAP suppressing the transcriptional activity of WT1 in SMCs. WTAP may thus also play a role in messenger RNA processing in mammalian cells, either dependent on or independent of its interaction with WT1.

Full Name
Wilms tumor 1 associated protein
References
  • Fukusumi Y, et al. (2008) Wtap is required for differentiation of endoderm and mesoderm in the mouse embryo. Dev Dyn. 237(3): 618-29.
  • Small TW, et al. (2007) Vascular biology and the sex of flies: regulation of vascular smooth muscle cell proliferation by wilms' tumor 1-associating protein. Trends Cardiovasc Med. 17(7): 230-4.
  • Small TW, et al. (2006) Wilms' tumor 1-associating protein regulates the proliferation of vascular smooth muscle cells. Circ Res. 99(12): 1338-46.
  • Rong Y, et al. (2006) Wilms' tumor 1 and signal transducers and activators of transcription 3 synergistically promote cell proliferation: a possible mechanism in sporadic Wilms' tumor. Cancer Res. 66(16): 8049-57.
  • WTAP cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    WTAP cDNA ORF Clone in Cloning Vector, Mouse MG52198-G
    WTAP cDNA ORF Clone, Mouse, untagged MG52198-UT
    WTAP Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG52198-ACGLN
    WTAP cDNA ORF Clone, Mouse, C-GFPSpark® tag MG52198-ACG
    WTAP cDNA ORF Clone, Mouse, N-GFPSpark® tag MG52198-ANG
    WTAP cDNA ORF Clone, Mouse, C-OFPSpark® tag MG52198-ACR
    WTAP cDNA ORF Clone, Mouse, N-OFPSpark® tag MG52198-ANR
    WTAP cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG52198-CF
    WTAP cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG52198-NF
    WTAP cDNA ORF Clone, Mouse, C-His tag MG52198-CH
    WTAP cDNA ORF Clone, Mouse, N-His tag MG52198-NH
    WTAP cDNA ORF Clone, Mouse, C-HA tag MG52198-CY
    WTAP cDNA ORF Clone, Mouse, N-HA tag MG52198-NY
    WTAP cDNA ORF Clone, Mouse, C-Myc tag MG52198-CM
    WTAP cDNA ORF Clone, Mouse, N-Myc tag MG52198-NM
    WTAP qPCR Primer Pairs, Mouse MP202071

    WTAP cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    WTAP cDNA ORF Clone in Cloning Vector, Human HG12018-G
    WTAP cDNA ORF Clone, Human, untagged HG12018-UT
    WTAP cDNA ORF Clone, Human, C-GFPSpark® tag HG12018-ACG
    WTAP Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG12018-ACGLN
    WTAP cDNA ORF Clone, Human, N-GFPSpark® tag HG12018-ANG
    WTAP cDNA ORF Clone, Human, C-OFPSpark® tag HG12018-ACR
    WTAP cDNA ORF Clone, Human, N-OFPSpark® tag HG12018-ANR
    WTAP cDNA ORF Clone, Human, C-DDK (Flag®) tag HG12018-CF
    WTAP cDNA ORF Clone, Human, C-DDK (Flag®) tag HG12018-G-F
    WTAP cDNA ORF Clone, Human, N-DDK (Flag®) tag HG12018-NF
    WTAP cDNA ORF Clone, Human, C-His tag HG12018-CH
    WTAP cDNA ORF Clone, Human, C-HA tag HG12018-CY
    WTAP cDNA ORF Clone, Human, N-HA tag HG12018-NY
    WTAP cDNA ORF Clone, Human, C-Myc tag HG12018-CM
    WTAP cDNA ORF Clone, Human, N-Myc tag HG12018-NM
    WTAP qPCR Primer Pairs, Human HP101554
    info info
    添加购物车成功! 添加购物车失败!