( We provide with GSK3B qPCR primers for gene expression analysis, HP100137 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10044-ACG|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10044-ACR|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG10044-ANG|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG10044-ANR|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10044-CF|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10044-CH|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10044-CM|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10044-CY|
|人 GSK3B 基因ORF全长cDNA(克隆载体)||HG10044-M|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10044-NF|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10044-NH|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10044-NM|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10044-NY|
|人 GSK3B 基因ORF全长cDNA克隆(表达载体)||HG10044-UT|
GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It Contains 1 protein kinase domain, and is expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in GSK3B gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. It may be required for early embryo development and neuron differentiation.