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人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G (Codon Optimized) ORF mammalian expression plasmid, C-HA 标签

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RSV RSV-G cDNA Clone产品信息
NCBI注册码:
参考序列ORF长度:897bp
cDNA基因描述:Full length Clone DNA of Human RSV (subtype A, strain Long) glycoprotein G / RSV-G with C terminal HA tag.
分子别称:G, HRSVgp07
分子种属:RSV
载体:pCMV3-C-HA
质粒:pCMV3-RSV-G(A-long)-HA
限制性酶切位点:KpnI + XbaI (6kb + 0.94kb)
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with P20895.2.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
RSV RSV-G 基因质粒图
Human respiratory syncytial virus (RSV) (subtype A, strain Long) glycoprotein G / RSV-G (Codon Optimized) natural ORF mammalian expression plasmid, C-HA tag
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G (Codon Optimized) ORF mammalian expression plasmid, C-HA 标签 on other vectors
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40041-ACG1950
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP 标签VG40041-ACR1950
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G ORF mammalian expression plasmid (Codon Optimized), C-Flag 标签VG40041-CF1770
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G (Codon Optimized) ORF mammalian expression plasmid, C-His 标签VG40041-CH1770
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G (Codon Optimized) ORF mammalian expression plasmid, C-Myc 标签VG40041-CM1770
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G (Codon Optimized) ORF mammalian expression plasmid, C-HA 标签VG40041-CY1770
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G ORF mammalian expression plasmid (Codon Optimized)VG40041-G760
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G (Codon Optimized) ORF mammalian expression plasmid, N-Flag 标签VG40041-NF1770
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G (Codon Optimized) ORF mammalian expression plasmid, N-His 标签VG40041-NH1770
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40041-NM1770
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G (Codon Optimized) ORF mammalian expression plasmid, N-HA 标签VG40041-NY1770
人类呼吸道合胞病毒 hRSV (subtype A, strain Long) glycoprotein G / 人类呼吸道合胞病毒-G ORF mammalian expression plasmid (Codon Optimized)VG40041-UT1770
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研究背景

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. HRSV G protein is a type II glycoprotein of 289-299 amino acids (depending on the virus strain) with a signal/anchor hydrophobic domain and is extensively modified by the addition of both N-and O-linked oligosaccharides to achieve the mature form of 80-90 kDa. The C-terminal ectodomain of the G protein has a central region and four cysteines which are conserved in all HRSV isolates and have been proposed as the putative receptor binding site. The G protein mediates attachment of the virus to the host cell membrane by interacting with heparan sulfate, initiating the infection. As similar to mucins in amino acid compositions, the RSV G protein can interact with host CX3CR1, the receptor for the CX3C chemokine fractalkine, and thus modulates the immune response and facilitate infection. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors. Unlike the other paramyxovirus attachment proteins, HRSV-G lacks both neuraminidase and hemagglutinating activities.

参考资料
  • Martin-Gallardo A. et al., 1993, J Gen Virol. 74 : 453-8.
  • Jose AM. et al.,1997, J Gen Virol. 78: 2411-8.
  • Feldman SA. et al., 1999, J Virol. 73: 6610-7.
  • García-Beato R. et al., 2000, J Gen Virol. 81: 919-27.
  • Zlateva KT. et al., 2004, J Virol. 78: 4675-83.
  • Trento A. et al., 2006, J Virol. 80: 975-84.
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