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人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F (Codon Optimized) ORF mammalian expression plasmid, N-His 标签

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RSV RSV-F cDNA Clone产品信息
NCBI注册码:
参考序列ORF长度:1725bp
cDNA基因描述:Full length Clone DNA of Human RSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F with N terminal His tag.
分子别称:F, HRSVgp08
分子种属:RSV
载体:pCMV3-SP-N-His
质粒:pCMV3-His-RSV-F(A-RSS2)
限制性酶切位点:KpnI + XbaI (6kb + 1.74kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with P11209.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
RSV RSV-F 基因质粒图
Human respiratory syncytial virus (RSV) (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F (Codon Optimized) ORF mammalian expression plasmid, N-His 标签 on other vectors
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40037-ACG2070
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP 标签VG40037-ACR2070
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F ORF mammalian expression plasmid (Codon Optimized), C-Flag 标签VG40037-CF1890
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F (Codon Optimized) ORF mammalian expression plasmid, C-His 标签VG40037-CH1890
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F (Codon Optimized) ORF mammalian expression plasmid, C-Myc 标签VG40037-CM1890
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F (Codon Optimized) ORF mammalian expression plasmid, C-HA 标签VG40037-CY1890
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F ORF mammalian expression plasmid (Codon Optimized)VG40037-G920
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F (Codon Optimized) ORF mammalian expression plasmid, N-Flag 标签VG40037-NF1890
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F (Codon Optimized) ORF mammalian expression plasmid, N-His 标签VG40037-NH1890
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40037-NM1890
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F (Codon Optimized) ORF mammalian expression plasmid, N-HA 标签VG40037-NY1890
人类呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / 人类呼吸道合胞病毒-F ORF mammalian expression plasmid (Codon Optimized)VG40037-UT1890
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研究背景

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F0), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.

参考资料
  • Martin-Gallardo A. et al., 1993, J Gen Virol. 74 (3): 453-8.
  • Jose A M. et al., 1997, J Gen Virol. 78: 2411-8.
  • Feldman SA. et al., 1999, J Virol. 73 (8): 6610-7.
  • Zlateva K.T. et al., 2004, J Virol. 78 (9): 4675-83.
  • Trento A. et al., 2006, J Virol. 80 (2): 975-84.
  • Branigan P J. et al., 2006, J Gen Virol. 87 (2): 395-8.
  • Eckardt-Michel J. et al., 2008, J. Virol. 82: 3236-49.
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    货号: VG40037-NH
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    • Human respiratory syncytial virus (RSV) (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His tag
    • Rhesus CD3d/CD3 delta Gene Plasmid Map 5615
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