|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10756-ACG|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10756-ACR|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10756-CF|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10756-CH|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10756-CM|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10756-CY|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10756-NF|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10756-NH|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10756-NM|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10756-NY|
|人 SFRP2 基因ORF全长cDNA克隆(表达载体)||HG10756-UT|
The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1~5) that act as extracellular signaling ligands. Each SFRP is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. sFRP2 is a member of the SFRP family acting as soluble modulators of Wnt signaling and contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain.sFRP2 inhibites hypoxia induced endothelial cell apoptosis and increases endothelial cell migration. It prevents mesoderm specification and maintains the cells in the undifferentiated state. SFRP2 is also a novel stimulator of angiogenesis that stimulates angiogenesis via a calcineurin/NFAT pathway, thus is regarded as a favorable target for the inhibition of angiogenesis in solid tumors. Mouse sFRP2 is highly expressed in the eye and is also detected in heart and lung at low level.