SFRP2 cDNA ORF Clone, Human, N-His tag

Cat: HG10756-NH
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SFRP2 cDNA ORF Clone, Human, N-His tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
888 bp
描述
Full length Clone DNA of Human secreted frizzled-related protein 2 with N terminal His tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-SP-N-His
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SFRP2 cDNA ORF Clone, Human, N-His tag Alternative Names
FRP-2 cDNA ORF Clone, Human;SARP1 cDNA ORF Clone, Human;SDF-5 cDNA ORF Clone, Human
SFRP2 Background Information

The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1~5) that act as extracellular signaling ligands. Each SFRP is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. sFRP2 is a member of the SFRP family acting as soluble modulators of Wnt signaling and contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain.sFRP2 inhibites hypoxia induced endothelial cell apoptosis and increases endothelial cell migration. It prevents mesoderm specification and maintains the cells in the undifferentiated state. SFRP2 is also a novel stimulator of angiogenesis that stimulates angiogenesis via a calcineurin/NFAT pathway, thus is regarded as a favorable target for the inhibition of angiogenesis in solid tumors. Mouse sFRP2 is highly expressed in the eye and is also detected in heart and lung at low level.

Full Name
secreted frizzled-related protein 2
References
  • Hu E., et al.,(1998), Tissue restricted expression of two human Frzbs in preadipocytes and pancreas. Biochem. Biophys. Res. Commun. 247:287-293.
  • Blackshaw S., et al., (2004), Genomic analysis of mouse retinal development.PLoS Biol. 2:1411-1431.
  • Clark H.F., et al.,(2003), The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.Genome Res. 13:2265-2270.
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