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人 ICOS Ligand / B7-H2 / ICOSLG ELISA Pair Set

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Human ICOSLG 组成成分
包被抗体:0.5 mg/mL of mouse anti-Human ICOS Ligand / B7-H2 / ICOSLG monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
检测抗体:0.2 mg/mL of mouse anti-Human ICOS Ligand / B7-H2 / ICOSLG monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use.
标准品:Each vial contains 66 ng of recombinant Human ICOS Ligand / B7-H2 / ICOSLG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
Human ICOSLG 检测灵敏度
The minimum detectable dose of Human ICOS Ligand / B7-H2 / ICOSLG was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human ICOSLG 产品介绍
The Human ICOS Ligand / B7-H2 / ICOSLG ELISA Pair Set is for the quantitative determination of Human ICOS Ligand / B7-H2 / ICOSLG.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human ICOS Ligand / B7-H2 / ICOSLG coated on a 96-well plate. Standards and samples are added to the wells, and any Human ICOS Ligand / B7-H2 / ICOSLG present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human ICOS Ligand / B7-H2 / ICOSLG monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human ICOS Ligand / B7-H2 / ICOSLG present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human ICOSLG 储存方法
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human ICOSLG ELISA 配对抗体标准曲线
ICOSL/ICOS ligand/B7-h2 研究背景

Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.

Human ICOSL/ICOS ligand/B7-h2 参考资料
  • Flesch IE. (2002) Inducible costimulator-ligand (ICOS-L). J Biol Regul Homeost Agents. 16(3): 217-9.
  • Kajiwara K, et al. (2009) Expression and function of the inducible costimulator ligand B7-H2 in human airway smooth muscle cells. Allergol Int. 58(4): 573-83.
  • Wong SC, et al. (2009) Functional hierarchy and relative contribution of the CD28/B7 and ICOS/B7-H2 costimulatory pathways to T cell-mediated delayed-type hypersensitivity. Cell Immunol. 256(1-2): 64-71.
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