IFNAR1 Protein, Mouse, Recombinant (His Tag)

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IFNAR1 Protein, Mouse, Recombinant (His Tag): 产品信息

纯度
> 96 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to inhibit mIFNB1-mediated protection of L929 mouse fibroblast cells infected with vesicular stomatitis virus (VSV) to viral lysis.
The ED50 for this effect is typically 0.2-1μg/mL.
蛋白构建
A DNA sequence encoding the extracellular domain of mouse IFNAR1 (NP_034638.2) extracellular domain (Met 1-Thr 429) was expressed, with a polyhistidine tag at the C-terminus.
NP No.
表达宿主
HEK293 Cells
种属
Mouse
预测 N 端
Glu 27
分子量
The recombinant mouse IFNAR1 consists of 414 amino acids and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm IFNAR1 is approximately 55-60 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IFNAR1 Protein, Mouse, Recombinant (His Tag): 图片

Measured by its ability to inhibit mIFNB1-mediated protection of L929 mouse fibroblast cells infected with vesicular stomatitis virus (VSV) to viral lysis. The ED50 for this effect is typically 0.2-1μg/mL.

IFNAR1 Protein, Mouse, Recombinant (His Tag): 别称

CD118 Protein, Mouse; Ifar Protein, Mouse; Ifnar Protein, Mouse; Ifrc Protein, Mouse; Infar Protein, Mouse

IFNAR1 背景信息

Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.
全称
interferon (alpha, beta and omega) receptor 1
参考文献
  • Yan H, et al. (1996) Phosphorylated interferon-alpha receptor 1 subunit (IFNaR1) acts as a docking site for the latent form of the 113 kDa STAT2 protein. EMBO J. 15(5): 1064-74.
  • Richter MF, et al. (1998) Specific contribution of Tyk2 JH regions to the binding and the expression of the interferon alpha/beta receptor component IFNAR1. J Biol Chem. 273(38): 24723-9.
  • Abramovich C, et al. (1997) A protein-arginine methyltransferase binds to the intracytoplasmic domain of the IFNAR1 chain in the type I interferon receptor. EMBO J. 16(2): 260-6.
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