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大鼠 IGF1/IGF‑I/IGF-1 基因ORF全长cDNA(克隆载体)

  • Rat IGF1 Gene cDNA Clone (full-length ORF Clone)
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大鼠 IGF1 cDNA Clone产品信息
NCBI注册码:NM_001082479.1
参考序列ORF长度:462bp
cDNA基因描述:Full length Clone DNA of Rattus norvegicus insulin-like growth factor 1.
分子别称:Igf1
分子种属:Rat
载体:pUC19 Vector
质粒:cpUC19-ratIgf1
限制性酶切位点:
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:M13-47 and RV-M
( We provide with IGF1 qPCR primers for gene expression analysis, RP300467 )
启动子:
Application:
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
大鼠 IGF1 基因质粒图
Rat IGF1 Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Product nameProduct name
研究背景

IGF I, also known as mechano growth factor, somatomedin-C, IGF-I and IGF1, is a secreted protein which belongs to the?insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ) and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.

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货号: RG80503-U
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产品数据可能随批次变化,最新数据请详见随货说明书。
请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”