All IL-10 reagents are produced in house and quality controlled, including 8 IL-10 Antibody, 3 IL-10 ELISA, 52 IL-10 Gene, 1 IL-10 Lysate, 6 IL-10 Protein, 3 IL-10 qPCR. All IL-10 reagents are ready to use.
Recombinant IL-10 proteins are expressed by E. coli, HEK293 Cells with fusion tags as Native, N-His.
IL-10antibodies are validated with different applications, which are ELISA, ELISA(Cap), WB.
IL-10cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each IL-10 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
IL-10ELISA Kit are quality controlled by 8 internation QC standard which guarantee every ELISA Kit with high quality.
IL-10 is a anti-inflammatory cytokine which belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.