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人 IL6R / CD126 蛋白 (His 标签)

  • Human IL6R / CD126 Protein (His Tag) SDS-PAGE
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 IL6R 蛋白产品信息
分子别称:CD126,gp80,IL-6R,IL-6R-1,IL-6RA,IL6Q,IL6RA,IL6RQ
蛋白构建:A DNA sequence encoding the extracellular domain of human IL6R (NP_000556.1) corresponding to amino acid (Met 1-Pro 365) was expressed with a C-terminal polyhistidine tag.
分子种属:Human
表达宿主:Human Cells
运输说明:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
 IL6R 蛋白质控检测
纯度:> 90 % as determined by SDS-PAGE
生物活性:1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/mL (100μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/ml.
2. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 20-80 ng/ml.
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
稳定性:Samples are stable for up to twelve months from date of receipt at -70℃
预测N端:Leu 20
分子量:The soluble form of recombinant human IL6R consists of 357 amino acids and has a predicted molecular mass of 40 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 60-65 kDa due to glycosylation.
缓冲液:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
 IL6R 蛋白使用指导
储存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 IL6R 蛋白SDS-PAGE
Human IL6R / CD126 Protein (His Tag) SDS-PAGE

IL6R 相关信号通路

    Other IL6R 蛋白 Products
    10398-H08H
    IL6R/IL-6R/CD126 研究背景

    Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

     IL6R/IL-6R/CD126 参考资料
  • Barill S, et al. (2000) The role of interleukin-6 and interleukin-6/interleukin-6 receptor-alpha complex in the pathogenesis of multiple myeloma. Eur Cytokine Netw. 11(4): 546-51.
  • Kang KW, et al. (2007) Novel role of IL-6/SIL-6R signaling in the expression of inducible nitric oxide synthase (iNOS) in murine B16, metastatic melanoma clone F10.9, cells. Free Radic Biol Med. 42(2): 215-27.
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    货号: 10398-H08H-50
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    请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”