IL17 Protein, Mouse, Recombinant

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IL17 Protein, Mouse, Recombinant: 产品信息

纯度
> 97 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
1. Measured by its binding ability in a functional ELISA.
2. Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The ED50 for this effect is 0.15-0.6 ng/mL.
蛋白构建
A DNA sequence encoding the mature form of mouse IL17a (Q62386) (Thr 22-Ala158) was expressed and purified.
NCBI Reference No.
表达宿主
E. coli
种属
Mouse
预测 N 端
Met
分子量
The recombinant mouse IL17a consists of 138 amino acids and predicts a molecular mass of 15.5 KDa. It migrates as an approximately 16 KDa band in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL17 Protein, Mouse, Recombinant: 图片

IL17 Protein, Mouse, Recombinant: 别称

Ctla-8 Protein, Mouse; Ctla8 Protein, Mouse; IL-17 Protein, Mouse; IL-17A Protein, Mouse; Il17 Protein, Mouse

IL17 背景信息

IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
全称
interleukin 17A
参考文献
  • Andoh A, et al. (2002) IL-17 selectively down-regulates TNF-alpha-induced RANTES gene expression in human colonic subepithelial myofibroblasts. J Immunol. 169(4):1683-7.
  • Kotake S, et al. (1999) IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest. 103(9):1345-52.
  • Laan M, et al. (1999) Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J Immunol. 162(4):2347-52.
  • Shin HC, et al. (1999) Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclic AMP-dependent signal transduction pathway. Cytokine. 10(11):841-50.
  • Psychological stress promotes neutrophil infiltration in colon tissue through adrenergic signaling in DSS-induced colitis model
    Author
    Deng, Q;Chen, H;Liu, Y;Xiao, F;Guo, L;Liu, D;Cheng, X;Zhao, M;Wang, X;Xie, S;Qi, S;Yin, Z;Gao, J;Chen, X;Wang, J;Guo, N;Ma, Y;Shi, M;
    Year
    2016
    Journal
    Brain Behav. Immun.
    Application
    cell-based
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