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人 ITGA1/Integrin alpha 1 基因ORF全长cDNA(克隆载体)

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     ITGA1 cDNA Clone产品信息
    NCBI注册码:NM_181501.1
    参考序列ORF长度:3537
    cDNA基因描述:ORF Clone of Homo sapiens integrin, alpha 1 DNA.
    分子别称:VLA1, CD49a
    分子种属:Human
    载体:pGEM-T Vector
    限制性酶切位点:
    Tag序列:
    序列信息:Same as Gene Bank Ref. ID sequence with the nucleotide 3482-3484 TGA (Stop codon) deletion mutation and the point mutation 276 T/C not causing the amino acid variation.
    测序引物:( We provide with ITGA1 qPCR primers for gene expression analysis, HP102136 )
    抗生素(大肠杆菌):Ampicillin
    运输方式:Each tube contains approximately 10 μg of lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
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    Datasheet & Documentation

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