Mouse Anti-Human IgG-Fc Secondary Antibody, Mouse MAb

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Mouse Anti-Human IgG-Fc Secondary Antibody, Mouse MAb (Mouse Monoclonal 抗体) 产品信息

产品名称
Mouse Anti-Human IgG-Fc Secondary Antibody, Mouse MAb
经验证的应用
ELISA
交叉反应
Reacts with: Human
特异性
Human IgG1
No cross-reactivity with Human IgM in ELISA
免疫原
Recombinant Human IgG Fc
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, reconbinant human IgG Fc.This antibody was purified by Protein A affinity chromatography .
来源
Monoclonal Mouse IgG1 Clone #3F4G4
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Mouse Anti-Human IgG-Fc Secondary Antibody, Mouse MAb (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
ELISA 1:5000-1:20000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Mouse Anti-Human IgG-Fc Secondary Antibody, Mouse MAb: 别称

Anti-IgG1 Fc Antibody; Anti-Ighg1 Antibody

IgG1 背景信息

As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.
参考文献
  • Carosella ED. et al., 1988, Cell Immunol. 112: 262-70.
  • Eckle I. et al., 1990, Clin Exp Immunol. 81: 352-6.
  • Lorriaine C. et al., 1995, J Biol Chem. 270:8164-71.
  • Gomez-Guerrero C. et al., 2000, J Immunol. 164: 2092-101.
  • Sprague ER. et al., 2004, J Biol Chem. 14: 14184-93.
  • Anthony RM. et al., 2008, Science. 320: 373-6.
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