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甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-HA 标签

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H5N1 HA cDNA Clone产品信息
NCBI注册码:
参考序列ORF长度:1704bp
cDNA基因描述:Full length Clone DNA of Influenza A H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin with C terminal HA tag.
分子别称:HA, Hemagglutinin
分子种属:H5N1
载体:pCMV3-C-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with ADF83651.1.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-HA 标签 on other vectors
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40022-ACG2070
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP 标签VG40022-ACR2070
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized), C-Flag 标签VG40022-CF1890
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-His 标签VG40022-CH1890
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-Myc 标签VG40022-CM1890
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-HA 标签VG40022-CY1890
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized)VG40022-G920
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-Flag 标签VG40022-NF1890
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His 标签VG40022-NH1890
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40022-NM1890
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-HA 标签VG40022-NY1890
甲型流感 H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized)VG40022-UT1890
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研究背景

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

参考资料
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37
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