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甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, N-His 标签

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H9N2 NA cDNA Clone产品信息
NCBI注册码:KF188368.1
参考序列ORF长度:1410bp
cDNA基因描述:Full length Clone DNA of Influenza A H9N2 (A/Chicken/Hong Kong/G9/1997) Neuraminidase with N terminal His tag.
分子别称:Neuraminidase, NA
分子种属:H9N2
载体:pCMV3-SP-N-His
质粒:
限制性酶切位点:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, N-His 标签 on other vectors
甲型流感 H9N2 (A/Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, C-GFPSpark 标签VG40034-ACG1950
甲型流感 H9N2 (A/Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, C-OFPSpark / RFP 标签VG40034-ACR1950
甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, C-Flag 标签VG40034-CF1770
甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, C-His 标签VG40034-CH1770
甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, C-Myc 标签VG40034-CM1770
甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, C-HA 标签VG40034-CY1770
甲型流感 H9N2 (A/Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid (Codon Optimized)VG40034-G760
甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, N-Flag 标签VG40034-NF1770
甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, N-His 标签VG40034-NH1770
甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, N-Myc 标签VG40034-NM1770
甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid, N-HA 标签VG40034-NY1770
甲型流感 H9N2 (Chicken/Hong Kong/G9/1997) 神经氨酸酶 (Neuraminidase / NA) natural ORF mammalian expression plasmidVG40034-UT1770
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研究背景

Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell.

Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our own cell surface proteins.

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. Monoclonal or polyclonal antibody can be raised with protein based antigen or peptide based antigen. Antibody raised with protein based antigen could have better specificity and/or binding affinity than antibody raised with peptide based antigen, but cost associated with the recombinant protein antigen is usually higher. Anti influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection.

Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.

参考资料
  • Sardet C., et al.,(1989), Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter. Cell 56:271-280.
  • Sardet C., et al., (1990), Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.Science 247:723-726.
  • Tse C.-M., et al.,(1991), Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.EMBO J. 10:1957-1967.
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