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甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), C端HA标签

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H9N2 NA cDNA Clone产品信息
NCBI注册码:
参考序列ORF长度:1404bp
cDNA基因描述:Full length Clone DNA of Influenza A H9N2 (A/Hong Kong/1073/99) Neuraminidase with C terminal HA tag.
分子别称:Neuraminidase, NA
分子种属:H9N2
载体:pCMV3-C-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with NP_859038.1.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), C端HA标签 on other vectors
甲型流感 H9N2 (A/Hong Kong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (表达载体)(密码子优化), C端GFPSpark标签VG40040-ACG1950
甲型流感 H9N2 (A/Hong Kong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), C端OFPSpark标签VG40040-ACR1950
甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), C端FLAG标签VG40040-CF1770
甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), C端His标签VG40040-CH1770
甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), C端Myc标签VG40040-CM1770
甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), C端HA标签VG40040-CY1770
甲型流感 H9N2 (A/Hong Kong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化)VG40040-G760
甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), N端FLAG标签VG40040-NF1770
甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), N端His标签VG40040-NH1770
甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), N端Myc标签VG40040-NM1770
甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), N端HA标签VG40040-NY1770
甲型流感 H9N2 (HongKong/1073/99)神经氨酸酶 (Neuraminidase / NA) 基因全长ORF克隆 (密码子优化) (表达载体), 无标签VG40040-UT1770
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研究背景

Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell.

Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our own cell surface proteins.

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. Monoclonal or polyclonal antibody can be raised with protein based antigen or peptide based antigen. Antibody raised with protein based antigen could have better specificity and/or binding affinity than antibody raised with peptide based antigen, but cost associated with the recombinant protein antigen is usually higher. Anti influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection.

Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.

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货号: VG40040-CY
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