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甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid (Codon Optimized)

  • Influenza A H9N2 (HongKong/1073/99) Neuraminidase / NA Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, untagged
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H9N2 NA cDNA Clone产品信息
NCBI注册码:
参考序列ORF长度:1404bp
cDNA基因描述:Full length Clone DNA of Influenza A H9N2 (HongKong/1073/99) Neuraminidase.
分子别称:Neuraminidase, NA
分子种属:H9N2
载体:pCMV3-untagged
质粒:pCMV3-H9N2-HK-99-NA
限制性酶切位点:HindIII + XbaI (6.1kb + 1.4kb)
Tag序列:
序列信息:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with NP_859038.1.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
H9N2 NA 基因质粒图
Influenza A H9N2 (HongKong/1073/99) Neuraminidase / NA Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, untagged
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid (Codon Optimized) on other vectors
甲型流感 H9N2 (A/Hong Kong/1073/99) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40040-ACG1950
甲型流感 H9N2 (A/Hong Kong/1073/99) 神经氨酸酶 (Neuraminidase / NA) (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP 标签VG40040-ACR1950
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid (Codon Optimized), C-Flag 标签VG40040-CF1770
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) (Codon Optimized) ORF mammalian expression plasmid, C-His 标签VG40040-CH1770
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) (Codon Optimized) ORF mammalian expression plasmid, C-Myc 标签VG40040-CM1770
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) (Codon Optimized) ORF mammalian expression plasmid, C-HA 标签VG40040-CY1770
甲型流感 H9N2 (A/Hong Kong/1073/99) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid (Codon Optimized)VG40040-G760
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) (Codon Optimized) ORF mammalian expression plasmid, N-Flag 标签VG40040-NF1770
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) (Codon Optimized) ORF mammalian expression plasmid, N-His 标签VG40040-NH1770
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40040-NM1770
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) (Codon Optimized) ORF mammalian expression plasmid, N-HA 标签VG40040-NY1770
甲型流感 H9N2 (HongKong/1073/99) 神经氨酸酶 (Neuraminidase / NA) ORF mammalian expression plasmid (Codon Optimized)VG40040-UT1770
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研究背景

Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell.

Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our own cell surface proteins.

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. Monoclonal or polyclonal antibody can be raised with protein based antigen or peptide based antigen. Antibody raised with protein based antigen could have better specificity and/or binding affinity than antibody raised with peptide based antigen, but cost associated with the recombinant protein antigen is usually higher. Anti influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection.

Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.

参考资料
  • Sardet C., et al.,(1989), Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter. Cell 56:271-280.
  • Sardet C., et al., (1990), Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.Science 247:723-726.
  • Tse C.-M., et al.,(1991), Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.EMBO J. 10:1957-1967.
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