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人 KLK3 / Kallikrein 3 ELISA Pair Set

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Human KLK3 组成成分
包被抗体:0.5 mg/mL of rabbit anti-KLK3 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating.
检测抗体:0.5 mg/mL mouse anti-KLK3 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-protector, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
标准品:Each vial contains 60 ng of recombinant KLK3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
Human KLK3 检测灵敏度
The minimum detectable dose of Human KLK3 / Kallikrein 3 was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human KLK3 产品介绍
The Human KLK3 / Kallikrein 3 ELISA Pair Set is for the quantitative determination of Human KLK3 / Kallikrein 3.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for KLK3 / Kallikrein 3 coated on a 96-well plate. Standards and samples are added to the wells, and any KLK3 / Kallikrein 3 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-KLK3 / Kallikrein 3 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of KLK3 / Kallikrein 3 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human KLK3 储存方法
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human KLK3 ELISA 配对抗体标准曲线
Human KLK3 参考资料
  • Koistinen H, et al. (2008) Development of peptides specifically modulating the activity of KLK2 and KLK3. Biol Chem. 389(6): 633-42.
  • Yousef GM, et al. (2002) Kallikreins, steroid hormones and ovarian cancer: is there a link? Minerva Endocrinol. 27(3): 157-66.
  • Parikh H, et al. (2011) Fine mapping the KLK3 locus on chromosome 19q13.33 associated with prostate cancer susceptibility and PSA levels. Hum Genet. 129(6): 675-85.
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    货号: SEK10771-5
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    • Human KLK3 / Kallikrein 3 ELISA standard curve
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