Anti-Galectin 3 / LGALS3 Antibody

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Anti-Galectin 3 / LGALS3 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-Galectin 3 / LGALS3 Antibody
经验证的应用
WB,IP
交叉反应
Reacts with: Human
特异性
Human Galectin 3 / LGALS3
免疫原
Recombinant Human LGALS3 / Galectin-3 protein (Catalog#10289-HNAE)
制备方法
Produced in rabbits immunized with purified, recombinant Human LGALS3 / Galectin-3 (rh LGALS3 / Galectin-3; Catalog#10289-HNAE; P17931; Met1-Ile250). LGALS3 / Galectin-3 specific IgG was purified by Human LGALS3 / Galectin-3 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Galectin 3 / LGALS3 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:1000-1:5000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Galectin 3 / LGALS3 Antibody (Rabbit Polyclonal 抗体) 图片

Anti-Galectin3 rabbit polyclonal antibody at 1:1000 dilution

Lane A: MCF7 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:30 kDa

Galectin3 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

Lane C:0.5 mg SW480 Whole Cell Lysate

Lane D:0.5 mg A431 Whole Cell Lysate

2 µL anti-Galectin3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Galectin3 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 30 kDa

Observed band size: 30 kDa

Anti-Galectin 3 / LGALS3 Antibody: 别称

Anti-CBP35 Antibody; Anti-GAL3 Antibody; Anti-GALBP Antibody; Anti-GALIG Antibody; Anti-L31 Antibody; Anti-LGALS2 Antibody; Anti-MAC2 Antibody

Galectin 3 / LGALS3 背景信息

LGALS3, a member of the lectin family, has an important role in tumor progression through inhibition of apoptosis.The galectin-3 gene (LGALS3) encodes a beta-galactose binding lectin. LGALS3 expression is associated with neoplastic transformation and with differentiation of monocytes to macrophages. Galectin-3 is a beta-galactoside-binding lectin which is involved in modulating inflammation and apoptosis. Elevated expression of galectin-3 has been demonstrated in synovium of rheumatoid arthritis (RA).
全称
lectin, galactoside-binding, soluble, 3
参考文献
  • Dumic J, et al. (2006) Galectin-3: an open-ended story. Biochim Biophys Acta. 1760(4): 616-35.
  • Sharma UC, et al. (2004) Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction. Circulation. 110(19): 3121-8.
  • Yan YP, et al. (2009) Galectin-3 mediates post-ischemic tissue remodeling. Brain Res. 1288: 116-24.
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