Anti-Latexin Antibody

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Anti-Latexin Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-Latexin Antibody
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Human
特异性
Human Latexin
No cross-reactivity with E.coli lysatein ELISA
免疫原
Recombinant Human Latexin protein (Catalog#10211-H07E)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Latexin (rh Latexin; Catalog#10211-H07E; NP_064554.3; Glu 2-Glu 222). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #8E1G6C1D4
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Latexin Antibody (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:5000-1:10000
IP 1-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Latexin Antibody (Mouse Monoclonal 抗体) 图片

Anti-LXN mouse monoclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:26 kDa

LXN was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-LXN mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-LXN mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25.7 kDa

Observed band size: 27 kDa

Anti-Latexin Antibody: 别称

Anti-ECI Antibody; Anti-TCI Antibody

Latexin 背景信息

Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI and LXN, is a cytoplasm protein which belongs to theprotease inhibitor I47 (latexin) family. It is highly expressed in heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite of the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. In addition, Latexin is also implicated in Alzheimer's disease.
全称
latexin
参考文献
  • Liu Q., et al.,(2000), Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor. Mol. Biol. Rep. 27:241-246.
  • Gauci S., et al., (2009), Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.nal. Chem. 81:4493-4501.
  • Muzny D.M., et al.,(2006), The DNA sequence, annotation and analysis of human chromosome 3.Nature 440:1194-1198.
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