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中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), C端HA标签

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MERS-CoV CoV-orf4a cDNA Clone产品信息
NCBI注册码:JX869059.2
参考序列ORF长度:330bp
cDNA基因描述:Full length Clone DNA of MERS-CoV (NCoV / Novel coronavirus) orf4a with C terminal HA tag.
分子别称:CoV-orf4a
分子种属:MERS-CoV
载体:pCMV3-C-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:Identical with the Gene Bank AFS88938 sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), C端HA标签 on other vectors
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), C端GFPSpark标签VG40083-ACG1950
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), C端OFPSpark标签VG40083-ACR1950
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), N端GFPSpark标签VG40083-ANG1950
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), N端OFPSpark标签VG40083-ANR1950
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), C端FLAG标签VG40083-CF1770
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), C端His标签VG40083-CH1770
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), C端Myc标签VG40083-CM1770
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), C端HA标签VG40083-CY1770
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆VG40083-G760
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), N端FLAG标签VG40083-NF1770
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), N端His标签VG40083-NH1770
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), N端Myc标签VG40083-NM1770
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), N端HA标签VG40083-NY1770
中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) orf4a 基因全长ORF克隆 (表达载体), 无标签VG40083-UT1770
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