methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10245-M-F

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methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
1437 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human methionyl aminopeptidase 2 with Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV2-FLAG
限制性酶切位点
KpnI + XhoI (5.4kb + 1.49kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
methionyl aminopeptidase 2 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
MAP2 cDNA ORF Clone, Human;MNPEP cDNA ORF Clone, Human;p67 cDNA ORF Clone, Human;p67eIF2 cDNA ORF Clone, Human
methionyl aminopeptidase 2 Background Information

METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.

Full Name
methionyl aminopeptidase 2
Research Areas
References
  • Bennett, et al. (1997) EPR Studies on the Mono- and Dicobalt (II)-Substituted Forms of the Aminopeptidase from Aeromonas proteolytica. Insight into the Catalytic Mechanism of Dinuclear Hydrolases. J Am Chem Soc. 119:1923-33.
  • Johansson, et al. (2008) Dicobalt II-II, II-III, and III-III Complexes as Spectroscopic Models for Dicobalt Enzyme Active Sites. Inorg Chem. 47:5079-92.
  • Bradshaw, et al. (2002) Aminopeptidases and angiogenesis. Essays Biochem. 38: 5-78.
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    Product Name Catalog#(PDF)
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    methionyl aminopeptidase 2 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    methionyl aminopeptidase 2 cDNA ORF Clone in Cloning Vector, Human HG10245-M
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, untagged HG10245-UT
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-GFPSpark® tag HG10245-ACG
    methionyl aminopeptidase 2 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10245-ACGLN
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