MIF cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10246-M-F

This product is not available, please contact order@sinobiological.com for relevant product if required.

Contact us:
215-583-7898
order@sinobiological.com
Distributors
All MIF Reagents
CRO Services

All MIF Reagents
MIF cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
348 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) DNA.
质粒
启动子
Enhanced CMV promoter
载体
pCMV2-FLAG
限制性酶切位点
KpnI + XhoI
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
MIF cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MIF cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
GIF cDNA ORF Clone, Human;GLIF cDNA ORF Clone, Human;MMIF cDNA ORF Clone, Human
MIF Background Information

Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is an ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence suggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important roles in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.

Full Name
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
References
  • Ohkawara T, et al. (2005) Pathophysiological roles of macrophage migration inhibitory factor in gastrointestinal, hepatic, and pancreatic disorders. J Gastroenterol. 40(2): 117-22.
  • Bach JP, et al.. (2009) The role of macrophage inhibitory factor in tumorigenesis and central nervous system tumors. Cancer. 115(10): 2031-40.
  • Rendon BE, et al.. (2009) Mechanisms of macrophage migration inhibitory factor (MIF)-dependent tumor microenvironmental adaptation. Exp Mol Pathol. 86(3): 180-5.
  • Grieb G, et al. (2010) Macrophage migration inhibitory factor (MIF): a promising biomarker. Drug News Perspect. 23(4): 257-64.
  • MIF cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    MIF cDNA ORF Clone in Cloning Vector, Mouse MG50066-M
    MIF cDNA ORF Clone, Mouse, untagged MG50066-UT
    MIF Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50066-ACGLN
    MIF cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50066-ACG
    MIF cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50066-ACR
    MIF cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50066-CF
    MIF cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50066-NF
    MIF cDNA ORF Clone, Mouse, C-His tag MG50066-CH
    MIF cDNA ORF Clone, Mouse, N-His tag MG50066-NH
    MIF cDNA ORF Clone, Mouse, C-HA tag MG50066-CY
    MIF cDNA ORF Clone, Mouse, N-HA tag MG50066-NY
    MIF cDNA ORF Clone, Mouse, C-Myc tag MG50066-CM
    MIF cDNA ORF Clone, Mouse, N-Myc tag MG50066-NM
    MIF qPCR Primer Pairs, Mouse MP200066

    MIF cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    MIF cDNA ORF Clone in Cloning Vector, Human HG10246-M
    MIF cDNA ORF Clone, Human, untagged HG10246-UT
    MIF Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10246-ACGLN
    MIF cDNA ORF Clone, Human, C-GFPSpark® tag HG10246-ACG
    MIF cDNA ORF Clone, Human, C-OFPSpark® tag HG10246-ACR
    MIF cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10246-CF
    MIF cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10246-NF
    MIF cDNA ORF Clone, Human, C-His tag HG10246-CH
    MIF cDNA ORF Clone, Human, N-His tag HG10246-NH
    MIF cDNA ORF Clone, Human, C-HA tag HG10246-CY
    MIF cDNA ORF Clone, Human, N-HA tag HG10246-NY
    MIF cDNA ORF Clone, Human, C-Myc tag HG10246-CM
    MIF cDNA ORF Clone, Human, N-Myc tag HG10246-NM
    MIF qPCR Primer Pairs, Human HP100296
    info info
    添加购物车成功! 添加购物车失败!