MMP-9 Matched ELISA Antibody Pair Set,Human

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MMP-9 Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
MMP-9 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human MMP-9
线性范围
31.25-2000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 0.5 mg/mL of mouse anti-MMP9 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 10327-MM06)
2. Detection Antibody 0.5 mg/mL of biotinylated rabbit anti-MMP9 monoclonal antibody. Dilute to a working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10327-R043)
3. Standard Each vial contains 70 ng of recombinant MMP9. Reconstitute standard powder with 1mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
4. Streptavidin-HRP 50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
产品概述
This MMP-9 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human MMP-9 . It contains Human MMP-9 capture antibody, Human MMP-9 detector antibody (Biotin) Streptavidin-HRP and a highly purified HEK293-expressed recombinant Human MMP-9 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.

MMP-9 Matched ELISA Antibody Pair Set,Human 图片

MMP-9 Matched ELISA Antibody Pair Set,Human: 别称

CLG4B Matched ELISA Antibody Pair Set, Human; GELB Matched ELISA Antibody Pair Set, Human; MANDP2 Matched ELISA Antibody Pair Set, Human; MMP-9 Matched ELISA Antibody Pair Set, Human

MMP-9 背景信息

Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions, such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. MMP9, also known as 92-kDa gelatinase B/type IV collagenase, is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodelling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils, and is particularly important in the pathogenesis of inflammatory, infectious, and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines, or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent of the delicate balance between MMPs and their inhibitors, excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence, regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. In addition, the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions clearly points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention.
全称
matrix metallopeptidase 9
参考文献
  • St-Pierre Y, et al. (2003) Emerging features in the regulation of MMP-9 gene expression for the development of novel molecular targets and therapeutic strategies. Curr Drug Targets Inflamm Allergy. 2(3): 206-15.
  • St-Pierre Y, et al. (2004) Regulation of MMP-9 gene expression for the development of novel molecular targets against cancer and inflammatory diseases. Expert Opin Ther Targets. 8(5): 473-89.
  • Chakrabarti S, et al. (2005) Matrix metalloproteinase-2 (MMP-2) and MMP-9 in pulmonary pathology. Exp Lung Res. 31(6): 599-621.
  • Decreased plasma levels of soluble CD18 link leukocyte infiltration with disease activity in spondyloarthritis
    Author
    Kragstrup, TW;Jalilian, B;Hvid, M;Kjærgaard, A;Ostgård, R;Schiøttz-Christensen, B;Jurik, AG;Robinson, WH;Vorup-Jensen, T;Deleuran, B;
    Year
    2014
    Journal
    Arthritis Res. Ther.
    Application
    ELISA
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