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小鼠 MST4/MST-4 基因ORF全长cDNA(克隆载体)

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    小鼠 MST4 cDNA Clone产品信息
    NCBI注册码:NM_133729.1
    参考序列ORF长度:1020bp
    cDNA基因描述:Full length Clone DNA of Mus musculus RIKEN cDNA 2610018G03 gene.
    分子别称:Mst4, RP23-245F8.1, 2610018G03Rik
    分子种属:Mouse
    载体:pGEM-T Vector
    质粒:pGEM-mMST4
    限制性酶切位点:
    Tag序列:
    序列信息:Identical with the Gene Bank Ref. ID sequence.
    测序引物:SP6 and T7 or M13-47 and RV-M
    ( We provide with MST4 qPCR primers for gene expression analysis, MP201046 )
    启动子:
    Application:
    抗生素(大肠杆菌):Ampicillin
    抗生素(哺乳动物细胞):
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
    Product nameProduct name
    研究背景

    MST4, also known as mammalian STE20-like protein kinase 4, is a novel member of the germinal center kinase subfamily of human Ste20-like kinases and is closely related to MST3. The 416 amino acid full-length MST4 contains a C-terminal regulatory domain and an N-terminal kinase domain, both of which are required for full activation of the kinase. MST4 is highly expressed in placenta, thymus, and peripheral blood leukocytes. MST4 specifically activates ERK but not JNK or p38 MAPK in transient transfected cells or in stable cell lines, and thus is biologically active in the activation of MEK/ERK pathway mediating cell growth and transformation. Further, MST4 kinase activity is stimulated significantly by epidermal growth factor receptor (EGFR) ligands, which are known to promote growth of certain cancer cells. Accordingly, MST4 have a potential role in signal transduction pathways involved in cancer progression. Three alternatively spliced isoform of MST4 have been isolated, and isoform 3 lacks an exon encoding kinase domain and may function as a dominant-negative regulator of the MST4 kinase.

    参考资料

    1. Qian, Z. et al., 2001, J Biol Chem. 276 :22439-45.

    2. Lin, JL. et al., 2001, Oncogene. 20: 6559-6569.

    3. Sung V, et al., 2003, Cancer research. 63: 3356-63.

    4. Ma, X. et al., 2007, Molecular biology of the cell. 18:1965-78.

    5. ten Klooster JP, et al., 2009, Developmental cell. 16:551-62.

    Size / Price
    货号: MG51088-G
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    库存In Stock
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