|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51014-ACG|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51014-ACR|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG51014-ANG|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG51014-ANR|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51014-CF|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51014-CH|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51014-CM|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51014-CY|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA(克隆载体)||MG51014-G|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51014-NF|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51014-NH|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51014-NM|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51014-NY|
|小鼠 ACP1 / LMW-PTP 基因ORF全长cDNA克隆(表达载体)||MG51014-UT|
The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine protein phosphatase family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. ACP1/LMW-PTP is an enzyme involved in platelet-derived growth factor-induced mitogenesis and cytoskeleton rearrangement. LMW-PTP is able to specifically bind and dephosphorylate activated PDGF receptor, thus modulating PDGF-induced mitogenesis. In vitro, LMW-PTP was found to efficiently dephosphorylate activated FcgammaRIIA and LAT, but not Syk or phospholipase Cgamma2. The overexpression of LMW-PTP inhibited activation of Syk downstream of FcgammaRIIA and reduced intracellular Ca(2+) mobilization. It been demonstrated that LMW-PTP is responsible for FcgammaRIIA dephosphorylation, and is implicated in the down-regulation of cell activation mediated by this ITAM-bearing immunoreceptor. In addition, ACP1 is a highly polymorphic phosphatase that is especially abundant in the central nervous system and is known to be involved in several signal transduction pathways.