|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51027-ACG|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51027-ACR|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG51027-ANG|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG51027-ANR|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51027-CF|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51027-CH|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51027-CM|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51027-CY|
|小鼠 AKT3 基因ORF全长cDNA(克隆载体)||MG51027-G|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51027-NF|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51027-NH|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51027-NM|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51027-NY|
|小鼠 AKT3 基因ORF全长cDNA克隆(表达载体)||MG51027-UT|
v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.