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小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), N-HA 标签

产品数据评论相关产品实验方法
Mouse ALOX5AP cDNA Clone产品信息
NCBI注册码:NM_009663.1
参考序列ORF长度:486bp
cDNA基因描述:Full length Clone DNA of Mus musculus arachidonate 5-lipoxygenase activating protein with N terminal HA tag.
分子别称:Flap
分子种属:Mouse
载体:pCMV3-N-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), N-HA 标签 on other vectors
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG52815-ACG1950
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG52815-ACR1950
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG52815-ANG1950
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG52815-ANR1950
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG52815-CF1770
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), C-His 标签MG52815-CH1770
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG52815-CM1770
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG52815-CY1770
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA(克隆载体)MG52815-G760
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG52815-NF1770
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), N-His 标签MG52815-NH1770
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG52815-NM1770
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG52815-NY1770
小鼠 ALOX5AP / 5-lipoxygenase-activating 蛋白 基因ORF全长cDNA克隆(表达载体)MG52815-UT1770
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Product nameProduct name
研究背景

Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP), also known as FLAP, belongs to the MAPEG family. ALOX5AP/FLAP is an essential partner of 5-LO for this process. The FLAP (ALOX5AP) gene has been linked to risk for myocardial infarction, stroke and restenosis, reigniting pharmaceutical interest in this target. It had been found that ALOX5AP/FLAP is a key enzyme in leukotriene formation, in both human pulmonary microvascular endothelial cells and a transformed human brain endothelial cell line. In addition, the protein FLAP has recently been identified as an emerging target in metabolic disease. In fact, FLAP is overexpressed in the adipose tissue of patients and experimental animals with obesity.

参考资料
  • Gonsalves CS, et al. (2010) Hypoxia-mediated expression of 5-lipoxygenase-activating protein involves HIF-1alpha and NF-kappaB and microRNAs 135a and 199a-5p. J Immunol. 184(7): 3878-88.
  • Zintzaras E, et al. (2009) Variants of the arachidonate 5-lipoxygenase-activating protein (ALOX5AP) gene and risk of stroke: a HuGE gene-disease association review and meta-analysis. Am J Epidemiol. 169(5): 523-32.
  • Evans JF, et al. (2008) What's all the FLAP about?: 5-lipoxygenase-activating protein inhibitors for inflammatory diseases. Trends Pharmacol Sci. 29(2): 72-8.
  • Bck M, et al. (2007) 5-Lipoxygenase-activating protein: a potential link between innate and adaptive immunity in atherosclerosis and adipose tissue inflammation. Circ Res. 100: 946-9.
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