|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51201-ACG|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51201-ACR|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51201-CF|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51201-CH|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51201-CM|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51201-CY|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51201-NF|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51201-NH|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51201-NM|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51201-NY|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA(克隆载体)||MG51201-U|
|小鼠 ApoE/Apolipoprotein E 基因ORF全长cDNA克隆(表达载体)||MG51201-UT|
Apolipoprotein E (ApoE) is a 34.2 kDa glycosylated protein with 299 amino acid residues. There are three isoforms in human (apoE2, apoE3, and apoE4) due to different amino acid residues at positions 112 and 158. ApoE is synthesized predominantly in the liver, but also by cells in the spleen, brain, lung, kidney, ovary, adrenal, and muscle tissues. Hepatic parenchyma cells are the main apoE producing cells in mammalian body, probably accounting for two thirds to three fourths of the plasma apoE . In the nervous system, apoE mRNA is present in neurons, astrocytes, ependymal cells, nonmyelinating Schwann cells, but not in microglia, oligodendroglia, choroidal cells, or myelinating Schwann cells. ApoE produced by mammalian cells exists in different forms, monomers, dimers, modified, unmodified, lipid-rich, and lipid-poor, and so forth. ApoE plays a double-role in immune responses. Both apoE containing lipoproteins and multimers of synthetic apoE peptides inhibited proliferation of cultured lymphocytes by inhibiting DNA synthesis and reducing phospholipid turnover in T cells. ApoE can also affect innate and acquired immune responses in vitro by its ability to suppress stimulation of cultured neutrophils. ApoE can bind lipopolysaccharide (LPS), attenuate the inflammatory response, and thus reduce LPS induced lethality. Injection of LPS stimulated higher expression of inflammatory cytokines like interleukin (IL)-1β, IL-12, and interferon-γ (IFN-γ), as well as IL-6.