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小鼠 B7-H5 / GI24 / VISTA HEK293 过表达细胞裂解液 (阳性对照)

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      细胞裂解液产品信息
    表达宿主:Human Cells
    产品介绍:Human Cell lysate that Mouse B7-H5 / GI24 / VISTA transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
    序列信息:A DNA sequence encoding the mouse C10orf54 (NP_083008.1) (Met1-Ala191) was expressed with a polyhistidine tag at the C-terminus.
    预测N端:Phe 33
    分子量:The recombinant mouse C10orf54 consists 170 amino acids and predicts a molecular mass of 19.2 kDa.
    分子种属:Mouse
      细胞裂解液使用指导
    制备方法:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
    裂解缓冲液:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
    质控:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
    稳定性:Samples are stable for up to twelve months from date of receipt.
    使用建议:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
    储存缓冲液:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
    储存方法:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
    应用小提示:Western blot (WB): Use at an assay dependent dilution.
    Other Applications: Not tested.
    Optimal dilutions/concentrations should be determined by the end user.
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    货号: 51550-M08HL-300
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