( We provide with CCL1 qPCR primers for gene expression analysis, MP200208 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50178-ACG|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50178-ACR|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50178-CF|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50178-CH|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50178-CM|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50178-CY|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA(克隆载体)||MG50178-M|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50178-NF|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50178-NH|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50178-NM|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50178-NY|
|小鼠 I-309/CCL1/TCA-3 基因ORF全长cDNA克隆(表达载体)||MG50178-UT|
CCL1 or chemokine (C-C motif) ligand 1, also known as I-309 or TCA-3, is a member of the chemokine (C-C motif) ligand family. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands (CCL)-1 to 28. I-309/CCL1/TCA-3 interacts with the G protein-linked transmembrane chemokine receptors CCR8, and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that I-309/CCL1/TCA-3 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, I-309/CCL1/TCA-3 was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. I-309/CCL1/TCA-3 induce significant levels of LTC4 from elicited eosinophils.