|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50192-ACG|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50192-ACR|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50192-CF|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50192-CH|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50192-CM|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50192-CY|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA(克隆载体)||MG50192-M|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50192-NF|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50192-NH|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50192-NM|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50192-NY|
|小鼠 VE-Cadherin/CD144 基因ORF全长cDNA克隆(表达载体)||MG50192-UT|
Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based diagnoses, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.