CD163 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag

Cat: MG51057-NF
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CD163 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag 基本信息
基因
种属
Mouse
NCBI 参考序列号
参考序列ORF长度
3366 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1056 T/C, 2052 T/C not causing the amino acid variation.
描述
Full length Clone DNA of Mouse CD163 antigen with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-SP-N-FLAG
限制性酶切位点
KpnI + XbaI (6kb + 3.34kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
CD163 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD163 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag Validated Images
Rhesus CD64/Fc gamma RI Gene Plasmid Map 5614
Mouse CD163 Gene Expression validated Image 14456
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
CD163 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag Alternative Names
CD163v2 cDNA ORF Clone, Mouse;CD163v3 cDNA ORF Clone, Mouse
CD163 Background Information
Full Name
CD163 molecule

CD163 cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
CD163 cDNA ORF Clone in Cloning Vector, Mouse MG51057-G
CD163 cDNA ORF Clone, Mouse, untagged MG51057-UT
CD163 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51057-ACG
CD163 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51057-ACGLN
CD163 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51057-ACR
CD163 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51057-CF
CD163 cDNA ORF Clone, Mouse, C-His tag MG51057-CH
CD163 cDNA ORF Clone, Mouse, N-His tag MG51057-NH
CD163 cDNA ORF Clone, Mouse, C-HA tag MG51057-CY
CD163 cDNA ORF Clone, Mouse, N-HA tag MG51057-NY
CD163 cDNA ORF Clone, Mouse, C-Myc tag MG51057-CM
CD163 cDNA ORF Clone, Mouse, N-Myc tag MG51057-NM
CD163 qPCR Primer Pairs, Mouse MP201015

CD163 cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
CD163 cDNA ORF Clone in Cloning Vector, Human HG10831-M
CD163 cDNA ORF Clone, Human, untagged HG10831-UT
CD163 cDNA ORF Clone, Human, C-GFPSpark® tag HG10831-ACG
CD163 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10831-ACGLN
CD163 cDNA ORF Clone, Human, C-OFPSpark® tag HG10831-ACR
CD163 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10831-CF
CD163 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10831-NF
CD163 cDNA ORF Clone, Human, C-His tag HG10831-CH
CD163 cDNA ORF Clone, Human, N-His tag HG10831-NH
CD163 cDNA ORF Clone, Human, C-HA tag HG10831-CY
CD163 cDNA ORF Clone, Human, N-HA tag HG10831-NY
CD163 cDNA ORF Clone, Human, C-Myc tag HG10831-CM
CD163 cDNA ORF Clone, Human, N-Myc tag HG10831-NM
CD163 qPCR Primer Pairs, Human HP100920
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