( We provide with PECAM1 qPCR primers for gene expression analysis, MP200085 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50408-ACG|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50408-ACR|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50408-CF|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50408-CH|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50408-CM|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50408-CY|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA(克隆载体)||MG50408-M|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50408-NF|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50408-NH|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50408-NM|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50408-NY|
|小鼠 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体)||MG50408-UT|
The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.