CD48 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag

Cat: MG50415-CF
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All CD48 Reagents
CRO Services
Product recommended

All CD48 Reagents
CD48 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag 基本信息
基因
种属
Mouse
NCBI 参考序列号
参考序列ORF长度
723 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Mouse CD48 antigen with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-FLAG
限制性酶切位点
HindIII + XbaI (6kb + 0.76kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
CD48 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD48 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Validated Images
Mouse CD48/Blast-1 Gene Expression validated Image 2162
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
Cynomolgus CD19/B4/CVID3 Gene Plasmid Map 5619
CD48 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Alternative Names
AI449234 cDNA ORF Clone, Mouse;AW610730 cDNA ORF Clone, Mouse;Bcm-1 cDNA ORF Clone, Mouse;BCM1 cDNA ORF Clone, Mouse;BLAST cDNA ORF Clone, Mouse;BLAST-1 cDNA ORF Clone, Mouse;BLAST1 cDNA ORF Clone, Mouse;MEM-102 cDNA ORF Clone, Mouse;Sgp-60 cDNA ORF Clone, Mouse;SLAMF2 cDNA ORF Clone, Mouse
CD48 Background Information

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD48 molecule
References
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
  • CD48 cDNA ORF Clone, Cynomolgus, Related Products

    Product Name Catalog#(PDF)
    CD48 cDNA ORF Clone in Cloning Vector, Cynomolgus CG90661-G
    CD48 cDNA ORF Clone, Cynomolgus, untagged CG90661-UT
    CD48 cDNA ORF Clone, Cynomolgus, C-GFPSpark® tag CG90661-ACG
    CD48 Lentiviral cDNA ORF Clone, Cynomolgus, C-GFPSpark® tag CG90661-ACGLN
    CD48 cDNA ORF Clone, Cynomolgus, C-OFPSpark® tag CG90661-ACR
    CD48 cDNA ORF Clone, Cynomolgus, C-DDK (Flag®) tag CG90661-CF
    CD48 cDNA ORF Clone, Cynomolgus, N-DDK (Flag®) tag CG90661-NF
    CD48 cDNA ORF Clone, Cynomolgus, C-His tag CG90661-CH
    CD48 cDNA ORF Clone, Cynomolgus, N-His tag CG90661-NH
    CD48 cDNA ORF Clone, Cynomolgus, C-HA tag CG90661-CY
    CD48 cDNA ORF Clone, Cynomolgus, N-HA tag CG90661-NY
    CD48 cDNA ORF Clone, Cynomolgus, C-Myc tag CG90661-CM
    CD48 cDNA ORF Clone, Cynomolgus, N-Myc tag CG90661-NM

    CD48 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    CD48 cDNA ORF Clone in Cloning Vector, Rat RG80286-G
    CD48 cDNA ORF Clone, Rat, untagged RG80286-UT
    CD48 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80286-ACG
    CD48 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80286-ACGLN
    CD48 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80286-ACR
    CD48 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80286-CF
    CD48 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80286-NF
    CD48 cDNA ORF Clone, Rat, C-His tag RG80286-CH
    CD48 cDNA ORF Clone, Rat, N-His tag RG80286-NH
    CD48 cDNA ORF Clone, Rat, C-HA tag RG80286-CY
    CD48 cDNA ORF Clone, Rat, N-HA tag RG80286-NY
    CD48 cDNA ORF Clone, Rat, C-Myc tag RG80286-CM
    CD48 cDNA ORF Clone, Rat, N-Myc tag RG80286-NM
    CD48 qPCR Primer Pairs, Rat RP300264

    CD48 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    CD48 cDNA ORF Clone in Cloning Vector, Mouse MG50415-M
    CD48 cDNA ORF Clone, Mouse, untagged MG50415-UT
    CD48 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50415-ACGLN
    CD48 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50415-ACG
    CD48 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50415-ACR
    CD48 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50415-NF
    CD48 cDNA ORF Clone, Mouse, C-His tag MG50415-CH
    CD48 cDNA ORF Clone, Mouse, N-His tag MG50415-NH
    CD48 cDNA ORF Clone, Mouse, C-HA tag MG50415-CY
    CD48 cDNA ORF Clone, Mouse, N-HA tag MG50415-NY
    CD48 cDNA ORF Clone, Mouse, C-Myc tag MG50415-CM
    CD48 cDNA ORF Clone, Mouse, N-Myc tag MG50415-NM
    CD48 qPCR Primer Pairs, Mouse MP200428

    CD48 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CD48 cDNA ORF Clone in Cloning Vector, Human HG10797-M
    CD48 cDNA ORF Clone, Human, untagged HG10797-UT
    CD48 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10797-ACGLN
    CD48 cDNA ORF Clone, Human, C-GFPSpark® tag HG10797-ACG
    CD48 cDNA ORF Clone, Human, C-OFPSpark® tag HG10797-ACR
    CD48 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10797-CF
    CD48 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10797-NF
    CD48 cDNA ORF Clone, Human, C-His tag HG10797-CH
    CD48 cDNA ORF Clone, Human, N-His tag HG10797-NH
    CD48 cDNA ORF Clone, Human, C-HA tag HG10797-CY
    CD48 cDNA ORF Clone, Human, N-HA tag HG10797-NY
    CD48 cDNA ORF Clone, Human, C-Myc tag HG10797-CM
    CD48 cDNA ORF Clone, Human, N-Myc tag HG10797-NM
    CD48 qPCR Primer Pairs, Human HP100711
    info info
    添加购物车成功! 添加购物车失败!